Biochem/physiol Actions

Reversible: yes

Product does not compete with ATP.

Primary TargetAkt

Cell permeable: yes

General description

A cell permeable, reversible, and substrate competitive phosphatidylinositol analog that inhibits the activation of Akt and select downstream substrates without decreasing phosphorylation of PDK-1 or other kinases downstream of Ras, such as MAPK. Also acts as a potent inducer of apoptosis and selectively kills a variety of cancer cell lines that contain high levels of active Akt. When used at a concentration of 10 µM, it is reported to activate p38α/SAPK2α MAP kinase. At 10 µM it will also inhibit PKA activity.

A phosphatidylinositol analog that inhibits the activation of Akt and selected downstream substrates without affecting the phosphorylation of PDK-1 and other downstream kinases. Decreases phosphorylation of Akt without affecting the total Akt level. Reported to be a potent inducer of apoptosis and to selectively kill a variety of cancer cell lines expressing high levels of active Akt. When used at a concentration of 10 µM, it is reported to activate p38α/SAPK2α MAP kinase. At 10 µM it will also inhibit PKA activity.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gills, J.J., et al. 2007. J. Biol. Chem.in press.Kozikowski, A.P., et al. 2003. J. Am. Chem. Soc.125, 1144.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)